Expression of a Fungal Cellulase Gene by \beta-glucanase Promoter of Streptococcus bovis

Authors: MEHMET SAİT EKİNCİ

Abstract: The promoter region of the §-glucanase gene was identified using a transcriptional fusion between the upstream region of the Streptococcus bovis \beta-glucanase gene and the celA gene. Using the transcriptional and protein localisation signals of the S. bovis \beta-glucanase gene, an in-frame tarnslational fusion between the end of the \beta-glucanase signal sequence and the ATG of the Neocallimastix patriciarum celA gene was constructed. The \beta-glucanase promoter-celA fusion was expressed in both E. coli and S. bovis. The activity of the protein produced was found to be cell-associated in E. coli, it but localised to the supernatant fraction of harvested cells of S. bovis. In this study for the first time we have demonstrated that the promoter of the gene from rumen bacteria can express a fungal gene.

Keywords: fungal cellulase, Streptococcus bovis, promoter, gene expression.

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