Availibility of use of Total Extracellular Proteins in SDS-PAGE for Characterization of Gram-Positive Cocci

Authors: SEFA CAN SAÇILIK, ÖZLEM OSMANAĞAOĞLU, UFUK GÜNDÜZ, CUMHUR ÇÖKMÜŞ

Abstract: There are no reports in the literature dealing with gram-positive cocci typing by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of extracellular proteins. In this study, Staphylococcus aureus, Staphylococcus warneri, Staphylococcus chromogenes, Micrococcus lysodeikticus and Micrococcus ssp. of the Micrococcaceae family, and Pediococcus halophilus, Streptococcus ssp. and Enterococcus faecalis of the Streptococcaceae family were characterized by SDS-PAGE of whole cell proteins (WCPs) and total extracellular proteins (TEPs). Comparison of SDS-PAGE of WCP profiles with banding patterns produced by that of TEPs provided additional criteria and characteristic banding patterns for the study of the characterization of gram-positive cocci. This method was found to be easy to use and interpret and provided a rapid, reproducible and discriminative method for typing gram-positive cocci.

Keywords: SDS-PAGE, gram-positive cocci, characterization.

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