Protein Profiles and Prevalence of Methicillin Resistant Staphylococcus aureus (MRSA) in Gülhane Military Academy Hospital in Turkey

Authors: SEFA CAN SAÇILIK, ÖZLEM OSMANAĞAOĞLU, ÖZGÜL KISA, AHMET BAŞUSTAOĞLU, CUMHUR ÇÖKMÜŞ

Abstract: The present study was undertaken to determine the relationship between Methicillin-Resistant Staphylococcus aureus (MRSA) strains isolated from various clinical specimens in Gülhane Military Medical Academy, in Ankara, Turkey. In 1997, at this hospital, the percentage of S. aureus among all nosocomial isolates was 20% and the percentage of MRSA among these S. aureus isolates was 51%. Both sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and Native-Page (N-PAGE) were used for typing MRSA isolates which have common antibiotic susceptibility test results. MRSA isolates were also evaluated according to the whole-cell proteins (WCPs) and filtrate proteins (FPs). Using these methods, we concluded that all of the MRSA strains are from the same clonal group. These results are consistent with the results of our previous study.

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