Electrophoretic Analysis of Candida albicans Isolates Collected From Turkish Hospitals by Native-Polyacrylamide Gel Electrophoresis (N-PAGE)

Authors: ÖZLEM OSMANAĞAOĞLU, SEFA CAN SAÇILIK, UFUK GÜNDÜZ, NURTEN ALTINLAR, CUMHUR ÇÖKMÜŞ

Abstract: Native-polyacrylamide gel electrophoresis (N-PAGE) was used to characterize Candida albicansstrains isolated from vaginal specimens of patients suffering from vaginitis in Ankara hospitals, Turkey. Three different protein band profiles were observed among these 38 C. albicansisolates when whole cell protein extracts of these strains were used in N-PAGE. 34 of the 38 isolates were shown to belong to a single group or clone with a dissemination percentage of 89, while three isolates belonged to another group with a percentage of 8. Furthermore only one C. albicans isolate was detected with a dissemination percentage of 3 and assigned to a third group. The results presented here also suggested that N-PAGE may be an efficacious method for differentiating between C. albicans strains.

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