Temporal and Spatial Determination of Germin Biosynthesis in Wheat Tissues

Authors: MAHMUT ÇALIŞKAN, ANDREW C. CUMING

Abstract: In the current study, the biosynthesis of germin was determined in the organs of wheat embryos during germination. The temporal and spatial determination of germin protein was carried out by employing an anti-germin sera in gel-blot analysis. Germin was detected in the coleoptile, roots and vascular transition region of and 6-day-old germinated wheat seedlings. It was shown that the leaf tissue acquires germin accumulation only at the late stage of germination. The pellet fraction of protein homogenate obtained from embryos at different ages revealed higher germin accumulation than the supernatant fraction from the same sources.

Keywords: Early wheat embryo development, Germin, Western blotting.

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