Production of Monoclonal Antibodies specific for Progesterone

Authors: FATMA ŞAHİNGÖZ YÜCEL, BEYAZIT ÇIRAKOĞLU

Abstract: Progesterone levels in milk and serum are indicators of pregnancy in cattle. The progesterone level reaches a peak on the 21 st and 22 nd days of pregnancy. Monoclonal antibodies specific to progesterone could be used for the immunodetection of milk and serum progesterone levels. We report here the development of hybrid cells prdoducing monoclonal antibodies specific for progesterone using hybridoma technology. Hybridoma cells secreting monoclonal antibodies against progesterone (MAM 2H11, MAM 6G6) were developed by fusion of the spleen cells of an immunized BALB/c mouse with FO (ATCC CRL 1646) mouse myeloma cells. These monoclonal antibodies were characterized for use in the development of diagnostic kits based on the enzyme-linked immunosorbent assay (ELISA) test system. ELISA tests showed that antibodies produced by 2 hybrids from 375 wells reacted specifically with progesterone. Monoclonal antibodies did not show any cross reaction with other steroid hormones.

Keywords: Immunodiagnosis, monoclonal antibody, progesterone, ELISA

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