Effect of Experimental Diabetes Mellitus on Plasma Lactate Dehydrogenase and Glutamic Oxaloacetic Transaminase Levels in Rabbits

Authors: İSMAİL ÇELİK, EŞREF YEĞİN, FEHMİ ODABAŞOĞLU

Abstract: Hyperglycemia was induced in 20 male, white, New Zealand rabbits (2-3 kg) (Oryctolagus cuniculus huxley) by alloxan (100 mg/kg body weight, I.V.) and maintained for 16 weeks without insulin treatment. Blood samples were taken from both diabetic and non-diabetic rabbits at the same time for measurement of plasma enzymes. Levels of glutamic oxalacetic transaminase (GOT) and lactate dehydrogenase (LDH) were determined after treatment. The levels of LDH and GOT were 155.6 ± 41.9 U/L and 47.0 ± 20.4 U/L in the control group respectively. These parameters were 286.9 ± 142.9 U/L and 93.9 ± 62.5 U/L in the diabetic group respectively. It was observed that LDH (p < 0.05) was significantly increased, whereas GOT was not significantly increased (p > 0.05) in the diabetic group. In conclusion, we agree that routine use of LDH measurement to monitor diabetic status may be useful, but the characteristic parameters of diabetes mellitus are better indicators.

Keywords: Diabetes mellitus, LDH, GOT, Rabbit

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