GA3, ABA and Cytokinin Production by Lentinus tigrinus and Laetiporus sulphureus Fungi Cultured in the Medium of Olive Oil Mill Waste

Authors: BİRGÜL ÖZCAN, S. FATİH TOPCUOĞLU

Abstract: The levels of endogenous form of free, bound and total-gibberellic acid (GA_3), abscisic acid (ABA) and cytokinin (zeatin) in culture medium were determined. The changes in dry weight of the mycelium, dependent on the culture periods, was examined through the use of white-rot fungus Lentinus tigrinus and brown-rot fungus Laetiporus sulphureus which were cultured in the medium of olive oil mill waste in a static culture. Spectrophotometric techniques were used to determine the amounts of endogenous GA_3, ABA and zeatin. As a result, fungi used in this study showed that they synthesized GA_3, ABA and zeatin as a primary or secondary metabolite and these plant hormones were found to be in free and bounded forms.

Keywords: Lentinus tigrinus, Laetiporus sulphureus, Fungi, Olive mill waste water, Gibberellic acid (GA_3), Abscisic acid (ABA), Zeatin, Plant hormones.

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