Comparison of different androgenesis protocols for doubled haploid plant production in ornamental pepper (Capsicum annuum L.)

Authors: ESİN ARI, TOLGA YILDIRIM, NEDİM MUTLU, SAADET BÜYÜKALACA, ÜMMÜ GÖKMEN, ECEHAN AKMAN

Abstract: Doubled haploid (DH) plant production plays an important role in pepper (Capsicum annuum L.) breeding for development of new varieties. However, information is lacking for DH plant production in pepper genotypes carrying ornamental value. The major goal of this study was to produce DH ornamental pepper lines having the mentioned value. Androgenic responses of 48 genotypes at the F₂ or F₃ generation were compared in each of three androgenesis protocols to determine the most effective method. Of the three protocols tested, anthers were placed on two different semisolid culture media and on a double-layer medium also called shed-microspore culture medium. The results revealed that the shed-microspore culture protocol was superior to both semisolid anther culture protocols. The average numbers of total and normal-looking embryos per bud of the most responsive genotype were 102.90 and 34.11, respectively. We report here, a total of 122 regenerated ornamental pepper plants with ornamental value; 63 DH, 52 haploid, and 7 mixoploid plants were produced in the present study. The spontaneous diploidization rate was 51.6% based on flow cytometry analyses. The results showed that the shed-microspore culture protocol could be used effectively for the development of DH lines in ornamental pepper breeding programs.

Keywords: Anther culture, breeding, Capsicum annuum L., doubled haploid, microspore embryogenesis, shed-microspore culture, ornamental pepper, ornamental plant

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