SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients

Authors: CİHAN TAŞTAN, BULUT YURTSEVER, GÖZDE KARAKUŞ, DERYA DİLEK KANÇAĞI, SEVDA DEMİR, SELEN ABANUZ, UTKU SEYİS, MÜLAZİM YILDIRIM, RECAİ KUZAY, ÖMER ELİBOL, SERAP ARBAK, MERVE AÇIKEL ELMAS, SELÇUK BİRDOĞAN, OSMAN UĞUR SEZERMAN, AYŞE SESİN KOCAGÖZ, KORAY YALÇIN, ERCÜMENT OVALI

Abstract: The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is caused by the severe acute respiratory syndrome coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. In May, over 150,000 cases in Turkey, and 5.5 million cases around the world have been declared. Due to the urgent need for a vaccine and antiviral drug, isolation of the virus is crucial. Here, we report 1 of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study provides an isolation and replication methodology,and cell culture tropism of the virus that will be available to the research communities.

Keywords: COVID-19, SARS-CoV-2, coronavirus

Full Text: PDF