Combined extract of Moringa oleifera and Centella asiatica modulates oxidative stress and senescence in hydrogen peroxide-induced human dermal fibroblasts

Authors: ELLY EZLINDA ABDUL HISAM, MOHD SALLEH ROFIEE, AINA MARDHIA KHALID, AHMAD FIRDAUS JALALUDDIN, MOHD IZWAN MUHAMMAD YUSOF, MUHD HANIS IDRIS, SALFARINA RAMLI, RICHARD JOHARI JAMES, WEE JACK YOENG, TEH LAY KEK, MOHD ZAKI SALLEH

Abstract: Moringa oleifera Lam. and Centella asiatica (L.) Urb. leaves have been previously reported to exhibit antioxidant activity. The objective of the present study is to determine the in vitro antioxidant activity of the combined extracts of M. oleifera and C. asiatica (TGT-PRIMAAGE) and its effect on hydrogen peroxide (H$_{2}$O$_{2}$)-induced oxidative stress in human dermal fibroblasts. TGT-PRIMAAGE acted on the mechanism of hydrogen transfer as it showed scavenging activity in the DPPH assay. This is due to the presence of phenolics and flavonoids in TGT-PRIMAAGE. TGT-PRIMAAGE effectively reduced cellular generation of reactive oxygen species induced by H$_{2}$O$_{2}$. The activities of superoxide dismutase and catalase were also increased in cells treated with TGT-PRIMAAGE. Treatment with TGT-PRIMAAGE showed significant reduction (P < 0.05) in the number of senescent cells. Significant reduction (P < 0.05) of malondialdehyde was also seen in cells treated with TGT-PRIMAAGE. The p53 protein level was reduced in TGT-PRIMAAGE-treated cells, which indicates its potential in protecting the cells from oxidative stress induced by H$_{2}$O$_{2}$.

Keywords: Moringa oleifera, Centella asiatica, senescence, oxidative stress, antioxidant

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