High-fat diet and glucose and albumin circadian rhythms' chronodisruption in rats

Authors: RAFAEL BRAVO, AMERICO CHINI, LIERNI UGARTEMENDIA, LOURDES FRANCO, MÓNICA MESA, ANA B. RODRIGUEZ, JAVIER CUBERO, CARMEN BARRIGA

Abstract: Obesity is one of the most widespread nutritional diseases in developed societies and it is considered a cardiovascular disease risk factor. The aim of the present work was to evaluate how a high-fat diet may influence the chronobiology of glucose and albumin circadian rhythms. Eighty-four male common Wistar rats were separated into two groups: a control group (n = 42) and a group fed a high-fat diet (n = 42); both for the control and the obesity-induced group we stablished 7 subgroups (6 rats per subgroup) to take blood samples at 0000, 0200, 0400, 1000, 1400, 1800, and 2200 hours. Glucose and albumin plasma levels were analyzed in blood samples and their circadian rhythms were evaluated through the cosinor method. Our results showed clear chronodisruption symptoms in both glucose and albumin oscillations, although these circadian disorders were more evident in glucose rhythms.

Keywords: Chronobiology, chronodisruption, obesity, high-fat diet, rats, glucose, albumin

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