Production of \alpha -Galactosidase using Bacillus stearothermophilusvar. caldiloactis C953

Authors: E. Esin KOCABAŞ, Mücahit DİZBAY

Abstract: An intracellular, thermostable a-galactosidase (a-D-galactoside galactohydrolase EC 3.2.1.22.) was produced from a strain of Bacillus stearothermophilus var. calidolactis C953. It was found that the presence of NH 4 Cl in the fermentation medium affected microbial growth and that optimum enzyme production occurred with peptone in various sources of nitrogen where NH 4 Cl was used as the main inorganic nitrogen source. After peptone, in decreasing order of enzyme productivity, came tryptone, soybean meal, soya-neutralized peptone and casamino acid. It was determined that, in various carbon sources, raffinose produced the highest a-galactosidase activity, followed by melibiose, D-galactose and molasses, and that in the medium with soybean meal extract the second highest level of activity occurred. Soybean oligosaccharides (raffinose-like sugars), because of their present low commercial value, could be of pratical use in the production of a-galactosidase.

Keywords: a-Galactosidase, Microbial enzyme production, Bacillus stearothermophilus

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