Determination of Antimicrobial and Antioxidant Activities of Turkish Endemic Salvia halophila Hedge

Authors: SEVİL ALBAYRAK, AHMET AKSOY, ERGİN HAMZAOĞLU

Abstract: In this study, the antioxidant activity of ethanol extract of Salvia halophila Hedge (Lamiaceae) was evaluated by 2 antioxidant assays, including phosphomolybdenum reduction and free radical scavenging activity. The ethanol extract showed free radical scavenging activity with IC_{50} of 67.73 \mug/mL in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant activity of the extract was found to be 84.87 ± 0.7 mg ascorbic acid equivalent (AAE)/g extract in phosphomolybdenum assay. In the addition to the antioxidant activity of the extract, the total phenolic content was measured. Total phenolic content of ethanol extract was found to be 8.21 ± 0.0 mg gallic acid equivalent (GAE)/g extract. Antimicrobial activity of the extract was examined against 13 bacteria and 2 yeasts by agar diffusion method. The ethanol extract of S. halophila had no effect against the tested yeasts. The ethanol extract showed antibacterial activity against all of the tested Gram (+) bacteria except Listeria monocytogenes at 1%, 2.5%, 5%, and 10% concentrations. The ethanol extract had no effect on the entire tested Gram (-) bacteria except Aeromonas hydrophila and Pseudomonas aeruginosa.

Keywords: Salvia halophila Hedge, antimicrobial and antioxidant activity, phenolic content, DPPH

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