Transformation Efficiency of R-Plasmids which were Isolated from Hospital Waste-water Bacteria

Authors: Sadık DİNÇER, Burhan ARIKAN, Ömer ÇOLAK, Hatice GÜVENMEZ

Abstract: As is widely known, hospital waste waters play a very important role in the increase and transmission of resistance in bacteria. A total of 52 transformation experiments were carried out using the CaCl2 method with R-plasmids which were isolated from hospital waste-water bacteria with plasmid-coded resistance to third-generation cephalosporins. Transformations were realised in 10 of the experiments in which 3 antibiotics were detected (19.2%). In 4 of the experiments 2 antibiotics were detected (7.7%), and in 18 of the experiments 1 antibiotic (34.6%). No transformation was obtained 20 of the experiments (38.4%).

Keywords: Bacterial transformation, Plasmids, Antibiotic resistance