The Effect of Plant Growth Promoting Rhizobacteria on Barley Seedling Growth, Nutrient Uptake, Some Soil Properties, and Bacterial Counts

Authors: RAMAZAN ÇAKMAKÇI, MESUDE FİGEN DÖNMEZ, ÜMMÜGÜLSÜM ERDOĞAN

Abstract: This study was conducted with barley in greenhouse conditions in order to investigate seed inoculation with 5 different N_2-fixing (Bacillus licheniformis RC02, Rhodobacter capsulatus RC04, Paenibacillus polymyxa RC05, Pseudomonas putida RC06, and Bacillus OSU-142) and 2 different phosphate-solubilising (Bacillus megaterium RC01 and Bacillus M-13) bacteria in comparison to control and mineral fertiliser (N and P) application. Among the strains used in the present study, 6 plant growth promoting rhizobacteria (PGPR) stimulated indole acetic acid (IAA) production and 3 of them stimulated phosphate solubilisation; all bacterial strains fixed N_2 and significantly increased the growth of barley. Available phosphate in soil was significantly increased by seed inoculation with Bacillus M-13 and B. megaterium RC01. Maximum NO_3-N was found in soil after inoculation with N_2-fixing Bacillus OSU-142, followed by P. polymyxa RC05 and R. capsulatus RC04. Total culturable bacteria count increased in all treatments with time, whereas N_2-fixing bacteria decreased with time, except with B. megaterium RC01 inoculation. The data suggest that seed inoculation of barley with plant PGPR increased root weight by 17.9%-32.1% as compared to the control, and increased shoot weight by 28.8%-54.2%, depending on the species. N_2-fixing bacterial inoculation significantly increased uptake of N, Fe, Mn, and Zn by barley. The production of hormones is suggested to be one of the mechanisms by which PGPR stimulate barley growth. Effective Bacillus species, such as OSU-142, RC07, M-13, P. polymyxa RC05, P. putida RC06, and R. capsulatus RC04, may be used in agriculture.

Keywords: Plant growth-promoting bacteria, IAA, nitrogen fixation, phosphate solubilisation, barley

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