In vitro Plantlet Regeneration from Mature Embryos of Linden (Tilia platyphyllos Scop.) and Multiplication of its Buds

Authors: ALİ ÖMER ÜÇLER, NURAY MOLLAMEHMETOĞLU

Abstract: In this study, the effects of different sucrose, BAP and Kinetin concentrations in MS medium on plantlet formation, bud formation on the plantlets and development of embryos isolated from the seeds of Tilia platyphyllos under in vitro conditions were investigated. In addition, the effects of different culture times on the development of embryos and plantlet formation were studied. The results indicated that the cultured embryos on the media supplemented with sucrose (10, 20, 30, 40, 50 and 60 g/l) developed plantlets with epicotyls and roots. The best results were obtained on the medium supplemented with 30 g/l of sucrose. Epicotyls of the embryos developing roots and shoots on the media with sucrose were transferred without roots to the medium of MS supplemented with BAP (0.2, 0.5, 1.0, 2.0 mg/l) or Kinetin (0.2, 0.5, 1.0, 2.0 mg/l). The average number of buds formed on the excised epicotyl explants on the media with BAP was higher than that with Kinetin. The best hypocotyl and root length growths were observed on the embryos which were cultured one month after harvesting the seeds on the medium supplemented with 30 g/l of sucrose. Some of the plantlets were then transferred into a mixture of 2 : 1 : 1 of peat, sand and perlite media, respectively, and were kept in greenhouse conditions. The results indicated that it is possible to germinate the embryos of the mature linden seeds on media supplemented with sucrose and then by using plantlet epicotyls taken into culture, multiplication of the buds can be realised.

Keywords: Tilia platypyllos, embryo culture, mature embryo, tissue culture, plantlet regeneration

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