Screening of common bean (Phaseolus vulgaris L.) cultivars against root-lesion nematode species

Authors: MEHMET ALİ SÖĞÜT, FATMA GÜL GÖZE, TUFAN ÖNAL, ZÜBEYİR DEVRAN, MUHAMMET TONGUÇ

Abstract: In this study, screening of 15 Phaseolus vulgaris cultivars and genotypes (Zülbiye, Kınalı, Perla, Kwintus, Özayşe, Tokat Sırık, Musica, Şelale, Nadide, Gina, Serra, Karabağ, Funda, and Lepus) and 1 Pisum sativum cultivar (Araka) for host suitability to 4 root-lesion nematodes, Pratylenchus thornei, P. crenatus, P. neglectus, and P. penetrans, was carried out in 2010 and 2011 under controlled conditions. The experiment was set up in a completely randomized block design with 7 replicates. Approximately 1000 adults + juveniles + eggs of root-lesion nematodes in 10 mL of distilled water were inoculated around the base of each bean seedling. The experiments were harvested after a 10-week duration. Nematodes were extracted from soil and roots by modified Baermann funnel technique and counted under a light microscope. Common bean cultivars and genotypes showed differences in host suitability in the 4 root-lesion nematodes. Pratylenchus penetrans reproduced on Zülbiye, Kınalı, Nadide, Gold Nectar, Karabağ, and Funda. Additionally, P. penetrans had a reproduction index value of slightly higher than 1 on Tokat Sırık, Şelale, and Serra. However, Gina, Musica, Perla, Kwintus, and Özayşe showed poor host reaction to P. penetrans, and P. penetrans did not develop on pea cultivar Araka. Pratylenchus thornei multiplied on Zülbiye, Şelale, Kınalı, Lepus, Perla, Nadide, and Araka. The value of the reproduction index was in the range of 1.8-3.1, and there were no significant statistical differences among these cultivars and genotypes. Özayşe, Tokat Sırık, Gold Nectar, Kwintus, Serra, Gina, Funda, and Musica significantly reduced P. thornei populations (P < 0.05). Reproduction indices of P. neglectus and P. crenatus were extremely low in all bean cultivars.

Keywords: Common bean, Phaseolus vulgaris, Pratylenchus spp., root lesion nematodes

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