Actinidia deliciosa: a high-resolution mass spectrometric approach for the comprehensive characterization of bioactive compounds

Authors: MAYYA RAZGONOVA, ALEXSANDER BOYKO, YULIA ZINCHENKO, NADEZHDA TIKHONOVA, ANDREY SABITOV, ALEXANDER MIKHAILOVICH ZAKHARENKO, KIRILL GOLOKHVAST

Abstract: Actinidia deliciosa (A. Chev.) C.F. Liang & A.R& Ferguson, 1984 contains a large number of target analytes, which are biologically active compounds. HPLC-ion trap-MS/MS (tandem mass spectrometry) was used to identify target analytes in extracts of A. deliciosa (varieties Abbot, Alison, Bruno, Hayward, Monti), originating from the Adler's Branch of N.I. Vavilov All-Russian Institute of Plant Genetic Resources. Separately, the pulp of the kiwi fruit and its peel were analyzed for the composition of biologically active substances. The richest in the composition of active substances was indicators of the cultivar Monti. The results of initial studies revealed the presence of 56 compounds in the extracts of the peel of A. deliciosa and the presence of 64 compounds in the extracts of berry pulp of A. deliciosa. Forty-three compounds were identified for the first time in family Actinidiaceae. New identified metabolites belonged to 17 classes including 13 flavones, 2 flavonols, 2 flavan-3-ols, 5 stilbenes, 4 lignans, 2 carotenoids, 2 sterols, 3 terpenoids, etc.

Keywords: Actinidia deliciosa, ion trap, kiwifruit, polyphenolic compounds, tandem mass spectrometry

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